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Inhibiting lipid droplet biogenesis enhances host protection against hypervirulent Klebsiella pneumoniae infections

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Author(s)
Hui-Jung JungHyun Ah KimMiri HyunJi Yeon LeeYoung Jae KimSeong-Il SuhEun-Kyeong JoWon-Ki BaekJin Kyung Kim
Keimyung Author(s)
Kim, Hyun AhHyun, Mi RiLee, Ji Yeon
Department
Dept. of Internal Medicine (내과학)
Journal Title
Med Microbiol Immunol
Issued Date
2024
Volume
213
Issue
1
Keyword
HypervirulentKlebsiella pneumoniaeMacrophagesLipid dropletsmTOR
Abstract
Hypervirulent Klebsiella pneumoniae (hvKp), an emerging Kp subtype, has become a serious global pathogen. However, the information regarding host interactions and innate immune responses during hvKp infection is limited. Here, we found that hvKp clinical strains increased triacylglycerol synthesis, resulting in lipid droplets (LDs) formation via the mammalian target of rapamycin signaling pathway in RAW264.7 cells. Treatment with rapamycin, an inhibitor of this pathway, affected LDs formation and antimicrobial responses against clinical hvKp infections. In accordance with the role of LDs in modulating inflammation, the pharmacological inhibition of lipogenesis reduced proinflammatory cytokine expression during hvKp infections. In addition, inhibition of LDs formation using pharmacological inhibitors and knockdown of lipogenesis regulators decreased the intracellular survival of hvKp in macrophages. Moreover, inhibiting LDs biogenesis reduced mortality, weight loss, and bacterial loads in hvKp-infected mice. Collectively, these data suggest that LDs biogenesis is crucial in linking host immune responses to clinical hvKp infections.
Keimyung Author(s)(Kor)
김현아
현미리
이지연
Publisher
School of Medicine (의과대학)
Type
Article
ISSN
1432-1831
Source
https://link.springer.com/article/10.1007/s00430-024-00807-x
DOI
10.1007/s00430-024-00807-x
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/46031
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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