흰쥐 담즙울체간의 Plasma Membrance, Mitochondria 및 Microsome의 5'-Nucleotidase와 Gamma-Glutamyl Transpeptidase의 활성치

Abstract

Changes in the activities of the following have been studied over a period of forty two days following the ligation of common bile duct in rats: plasma membrane, microsomal, mitochondrial, nuclear and cytosolic 5'-nucleotidase and gamma-glutamyl transpeptidase of cholestatic liver. The activities of alkaline phosphatase in the subcellular fractions of cholestatic liver were also measured. The activities of microsomal and cytosolic 5'-nucleotidase in cholestatic liver markedly elevated between the second and the forty-second days of the operation. However, the plasma membrane, mitochondrial and nuclear5'-nucleotidase activities in the cholestatic liver showed a substantial decrease from three days to forty two days after the ligation of common bile duct. The activities of hepatic microsomal, cytosolic, plasma membrane, mitochondrial and nuclear gtamma-glutamyl transpeptidase drastically increased throughout the experiments. The activities of microsomal and cytosolic alkaline phosphatase in the cholestatic liver strikingly increased during the forty two days following the operation, but the plasma-membrane bound alkaline phosphatase in the cholestatic liver had a significant diminution after the ligation of common bile duct.