INHIBITORY EFFECTS OF GLUCOSAMINE ON LIPOPOLYSACCHARIDE-INDUCED ACTIVATION IN MICROGLIAL CELLS

Authors
Hyon-Ah YiSang-Doe YiByeong-Churl JangDae-Kyu SongDong-Hoon ShinKyo-Chul MunSang-Pyo KimSeong-Il SuhJae Hoon Bae
Department
Dept. of Neurology (신경과학); Dept. of Physiology (생리학); Dept. of Preventive Medicine (예방의학); Dept. of Microbiology (미생물학); Dept. of Pathology (병리학); Dept. of Biochemistry (생화학); Dept. of Molecular Medicine (분자의학); Brain Research Institute (뇌연구소)
Issue Date
2005
Citation
Clinical and Experimental Pharmacology and Physiology, Vol.32(12) : 1097-1103, 2005
ISSN
0305-1870
Abstract
SUMMARY 1. The aim of the present study was to investigate the effects of glucosamine on lipopolysaccharide (LPS)-induced cellular activation in microglia and to evaluate the inhibitory mechanisms involved. 2. Lipopolysaccharide (100 ng/mL) was used for the activation of primary cultured rat microglial or BV2 microglial cells. Changes in intracellular Ca 2+ levels and outward K + currents were measured using fura-2/AM and whole-cell patch-clamp methods, respectively. Lipopolysaccharide- induced expression of tumour necrosis factor (TNF)-  mRNA was analysed by reverse transcription–polymerase chain reaction. 3. Lipopolysaccharide transformed cell morphology into an amoeboid shape in vitro and induced microglial activation in vivo , as measured by immunohistochemical staining, but glucosamine inhibited this activation. Glucosamine also inhibited LPS-induced Ca 2+ influx, outward K + currents and TNF-  mRNA expression, which are typically representative of microglial activation. 4. The results suggest that the inhibitory mechanisms of glucosamine on LPS-induced microglial activation include inhibition of Ca 2+ influx and outward K + currents, as well as downregulation of the microglial activator gene TNF-  . Key words: glucosamine, lipopolysaccharide, microglial activation.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35452
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Neurology (신경과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Preventive Medicine (예방의학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Brain Research Institute (뇌연구소)
Keimyung Author(s)
이현아; 이상도; 송대규; 배재훈; 신동훈; 서성일; 김상표; 문교철; 장병철
Full Text
http://lps3.onlinelibrary.wiley.com.proxy.dsmc.or.kr/doi/abs/10.1111/j.1440-1681.2005.04305.x
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