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Freeze-dried bovine amniotic membrane as a cell delivery scaffold in a porcine model of radiation-induced chronic wounds

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Affiliated Author(s)
손대구김진희권선영
Alternative Author(s)
Son, Dae GuKim, Jin HeeKwon, Sun Young
Journal Title
Arch Plast Surg
ISSN
2234-6171
Issued Date
2021
Keyword
Amniotic membraneCell therapyTissue scaffoldWound healingExperimental animal model
Abstract
Background:
Locoregional stem cell delivery is very important for increasing the efficiency of cell therapy. Amnisite BA (Amnisite) is a freeze-dried amniotic membrane harvested from bovine placenta. The objective of this study was to investigate the retention of cells of the stromal vascular fraction (SVF) on Amnisite and to determine the effects of cell-loaded Amnisite in a porcine radiation-induced chronic wound model.

Methods:
Initially, experiments were conducted to find the most suitable hydration and incubation conditions for the attachment of SVF cells extracted from pig fat to Amnisite. Before seeding, SVFs were labeled with PKH67. The SVF cell-loaded Amnisite (group S), Amnisite only (group A), and polyurethane foam (group C) were applied to treat radiation-induced chronic wounds in a porcine model. Biopsy was performed at 10, 14, and 21 days post-operation for histological analysis.

Results:
Retaining the SVF on Amnisite required 30 minutes for hydration and 1 hour for incubation. A PKH67 fluorescence study showed that Amnisite successfully delivered the SVF to the wounds. In histological analysis, group S showed increased re-epithelialization and revascularization with decreased inflammation at 10 days post-operation.

Conclusions:
SVFs had acceptable adherence on hydrated Amnisite, with successful cell delivery to a radiation-induced chronic wound model.
Department
Dept. of Plastic Surgery (성형외과학)
Dept. of Radiation Oncology (방사선종양학)
Dept. of Pathology (병리학)
Publisher
School of Medicine (의과대학)
Citation
Daemyung Oh. (2021). Freeze-dried bovine amniotic membrane as a cell delivery scaffold in a porcine model of radiation-induced chronic wounds. Arch Plast Surg, 48(4), 448-456. doi: 10.5999/aps.2020.00997
Type
Article
DOI
10.5999/aps.2020.00997
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/43671
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. School of Medicine (의과대학) > Dept. of Plastic Surgery (성형외과학)
1. School of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학)
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