(–)-EPIGALLOCATECHIN GALLATE ATTENUATES GLUTAMATE-INDUCED CYTOTOXICITY VIA INTRACELLULAR CA2+ MODULATION IN PC12 CELLS

Authors
Jong-Hun LeeDae-Kyu SongChul-Ho JungDong-Hoon ShinJongWook ParkTaeg Kyu KwonByeong-Churl JangKyo-Cheol MunSang-Pyo KimSeong-Il SuhJae Hoon Bae
Department
Dept. of Psychiatry (정신건강의학); Dept. of Physiology (생리학); Dept. of Immunology (면역학); Dept. of Microbiology (미생물학); Dept. of Pathology (병리학); Dept. of Biochemistry (생화학); Dept. of Molecular Medicine (분자의학); Dept. of Preventive Medicine (예방의학); Brain Research Institute (뇌연구소)
Issue Date
2004
Citation
Clinical and Experimental Pharmacology and Physiology, Vol.31(8) : 530-536, 2004
ISSN
0305-1870
Abstract
SUMMARY 1. The effects of (–)-epigallocatechin gallate (EGCG), a green tea polyphenol, on glutamate-induced increases in intracellular Ca2+ concentrations ([Ca2+]i) and cytotoxicity in PC12 cells were investigated. 2. Changes in [Ca2+]i were measured using Fura-2/AM calcium indicator dye and cellular viabilities were determined by a viable cell count and a 3-(4,4-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide reduction assay. 3. Glutamate increased [Ca2+]i in PC12 cells in a dosedependent manner. (–)-Epigallocatechin gallate attenuated this glutamate (30 mmol/L)-induced [Ca2+]i increase and EGCG (50 mol/L) increased the viability of PC12 cells against glutamate-induced cytotoxicity. The EGCG effect was also found to be independent of its general anti-oxidant mechanism. In contrast, EGCG directly suppressed both N-methyl-Daspartate (50 mmol/L)- and kainate (20 mmol/L)-mediated Ca2+ influx, but not metabotropic receptor-mediated Ca2+ release. 4. These results suggest that EGCG reduces the glutamateinduced [Ca2+]i increase by attenuating ionotropic Ca2+ influx and that this promotes the viability of PC12 cells. Key words: (–)-epigallocatechin gallate, glutamate, intracellular calcium modulation.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35451
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Preventive Medicine (예방의학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Psychiatry (정신건강의학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Brain Research Institute (뇌연구소)
Keimyung Author(s)
정철호; 송대규; 배재훈; 권택규; 박종욱; 서성일; 김상표; 문교철; 장병철; 신동훈
Full Text
http://lps3.onlinelibrary.wiley.com.proxy.dsmc.or.kr/doi/abs/10.1111/j.1440-1681.2004.04044.x
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